Application

Flow cytometry

Immunoprecipitation: Effective; however because this antibody is an IgG2a, protein G or rabbit anti-mouse IgG is helpful for capture.

Immunohistochemistry/immunocytochemistry: The antibody recognizes alphaVbeta6 by immunohistochemistry and immunocytochemistry (acetone fix), but nuclear background staining limits usefulness in these techniques.


Then antibody is not function blocking and not effective for western blot.

Optimal working dilutions must be determined by end user.

Anti-Integrin β6 Antibody, clone R6G9, azide free is an antibody against Integrin β6 for use in FC & IP.

Immunogen

Murine NIH 3T3 cells stably transfected with the human (6 subunit

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Physical form

Format: Purified

Specificity

Monoclonal antibody MAB2075Z, raised by immunizing Balb/c mice with murine NIH 3T3 cells stably transfected with the human beta6 subunit, recognizes the human beta6 subunit by flow cytometry and is an excellent antibody for immunoprecipitation.